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Large Production System for Hyperpolarized129 Xe for Human Lung Imaging Studies

Rationale and Objectives

Hyperpolarized gases such as 129 Xe and 3 He have high potential as imaging agents for functional lung magnetic resonance imaging (MRI). We present new technology offering 129 Xe production rates with order-of-magnitude improvement over existing systems, to liter per hour at 50% polarization. Human lung imaging studies with xenon, initially limited by the modest quantity and quality of hyperpolarized gas available, can now be performed with multiliter quantities several times daily.

Materials and Methods

The polarizer is a continuous-flow system capable of producing large quantities of highly-polarized 129 Xe through rubidium spin-exchange optical pumping. The low-pressure, high-velocity operating regime takes advantage of the enhancement in the spin exchange rate provided by van der Waals molecules dominating the atomic interactions. The long polarizing column moves the flow of the gas opposite to the laser direction, allowing efficient extraction of the laser light. Separate sections of the system assure full rubidium vapor saturation and removal.

Results

The system is capable of producing 64% polarization at 0.3 L/hour Xe production rate. Increasing xenon flow reduces output polarization. Xenon polarization was studied as a function of different system operating parameters. A novel xenon trapping design was demonstrated to allow full recovery of the xenon polarization after the freeze-thaw cycle. Delivery methods of the gas to an offsite MRI facility were demonstrated in both frozen and gas states.

Conclusions

We demonstrated a new concept for producing large quantities of highly polarized xenon. The system is operating in an MRI facility producing liters of hyperpolarized gas for human lung imaging studies.

Although protons in living tissue achieve sufficient thermal polarization for standard magnetic resonance imaging (MRI) procedures, gaseous samples have three orders of magnitude lower density, making diagnostic imaging impractical. Similarly, the low body tissue density and susceptibility mismatched interfaces inside the lungs present a challenge to proton MRI of the lungs. Hyperpolarized gases are polarized by alternative methods, such as spin-exchange optical pumping (SEOP) ( ) or metastability exchange optical pumping ( ), to extremely high values to compensate for the density difference, and they offer great potential as imaging agents. They can be introduced into the body noninvasively by breathing and imaged with no background. Two noble gases, 3 He and 129 Xe, have nuclear spin half and gyromagnetic ratios relative to that of the proton of 0.76 and 0.28, respectively. They present the advantage of having zero quadrupole moment and, as a consequence, long T 1 relaxation times after being polarized. Although the first MRI application of hyperpolarized noble gases involved 129 Xe ( ), thereafter the research community focused mainly on 3 He because of the well-established polarizing methods ( ). Relatively large quantities of hyperpolarized 3 He are produced with 50%–80% polarization using metastability-exchange optical pumping. 3 He in the gas state has a T 1 relaxation time that can reach days and even weeks ( ). As a beneficial consequence, 3 He can be distributed from a central polarizing site to MRI centers ( ). Practical limitations of MRI studies involving 3 He include its confinement to the lung gas space only ( ), because its solubility in blood and tissue is extremely low ( ). Also, its unnatural source on Earth, mainly coming from tritium radioactive decay, makes its future widespread clinical applicability questionable. In contrast, xenon is extracted from the atmosphere by partial distillation, with the desired 129 Xe isotope having significant 26.4% natural abundance. Commercial enrichment can raise that abundance by a factor of three or more. Xenon is soluble in blood with a large partition coefficient of ∼0.1 ( ) and readily diffuses throughout tissues. 129 Xe displays a nuclear magnetic resonance (NMR) spectrum with chemical shifts in resonance frequency that characterize its microscopic/molecular environment ( ). It offers longitudinal magnetization that survives several seconds to several tens of seconds in vivo ( ). The time and spatial dependence of its distribution in the body can reveal functional attributes ( ). Xenon biologic effects of anesthesia are well characterized and well tolerated ( ).

Spin-exchange optical pumping ( ) relies on the unpaired electron of the alkali atoms, such as Rb, which is polarized through resonant interaction with circularly polarized light provided by a continuous laser source. Subsequently, the polarization is transferred through a Fermi-contact dipole-dipole interaction to the noble gas nucleus. The presence of nitrogen is required in the gas mixture for quenching the fluorescence of the excited alkali vapor atoms ( ). Natural helium ( 4 He) is also commonly used as a buffer gas to increase the overall pressure inside the cell in order to take advantage of the pressure broadening effects on the Rb D1 absorption line ( ).

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PXe=PAγSEγSE+Γ[1−e−t(γSE+Γ)] P

Xe

=

P

A

γ

SE

γ

SE

+

Γ

[

1

e

t

(

γ

SE

+

Γ

)

]

where P A is the alkali vapor polarization, Γ is the noble gas nuclear spin-destruction rate, and γ SE is the spin-exchange rate between the alkali atoms and gas nuclei. Similar to the alkali, the noble gas nuclei can lose their polarization interacting with the container walls or with the other atoms in the composition. Xenon polarization can never exceed the rubidium polarization. Moreover, to approach the Rb polarization, the noble gas has to be in contact with the alkali vapor for several spin-up e-folding times. The spin-up time is defined as (γ SE + Γ) –1 , and for Rb-Xe pairs under our operating conditions is on the order of seconds. This implies that high polarizations of xenon can be achieved only if low concentrations of xenon are in contact with highly polarized rubidium for characteristically long times: the challenge of producing large quantities of highly polarized xenon.

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Materials and methods

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Figure 1, (a) University of New Hampshire polarizer schematic: 1) xenon/nitrogen/helium gas mixture input; 2) Rb metal puddles vapor sources; 3) spin-exchange optical pumping hot region; 4) Rb condensing cold region; 5) liquid cooled jacket; 6) gas transport down-tube; 7) polarization measurement nuclear magnetic resonance system; 8) Xe freeze-out helix located inside the 0.3 T magnet-box; 9) gas exhaust; 10) oil heated oven/bath; 11) laser entrance; 12) laser spectrum absorption monitor (spectrometer); 13) B o field coils. The solid arrows show the gas trajectory in the system. (b) Polarizing column with its main components.

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Polarizing Column

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B 0 Field

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Gas Flow System

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Figure 2, Gas flow system schematic: 1) connection to calibration volume; 2) purifier; 3) purifier bypass loop; 4) pressure gauge; 5) secondary vacuum pump; 6) thermal cell filling port; 7) down-tube; 8) freeze-out; 9) pressure gauge; 10) ballast gas input; 11) exhaust port to main vacuum pump. ⊗: valve; MFC: mass flow controller; PC: pressure controller.

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Laser and Optics

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Figure 3, (a) Schematic of the classical optical setup for circular polarizing laser light: a linear polarizing beam splitter cube followed by quarter wave plates on each component. (b) Schematic of the University of New Hampshire optical setup: starting with a half-circle fiber output, the transmitted and the reflected beams meet at the imaging point “O” to create a final circular beam.

Figure 4, (a) By using only five inputs of the seven available, the fiber output approaches the half-circle shape; (b) high magnification projected image of the combined beams; (c) final optical setup and the output beam shape at one meter away: the beam fills the 4-cm diameter target.

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Heating Oven

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Xenon Accumulation System

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Figure 5, (a) Classic cold-finger style freeze-out. Gas comes down through the middle tube and accumulates on the outside tube surface. (b) Novel helix design freeze-out: gas comes down through the helix and exits upwards through the middle tube. (c) Schematic of the freeze-out system: the xenon is accumulated uniformly inside the helix by raising the liquid nitrogen level in a controlled manner.

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Polarization Measurement

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SXeSp=PXePpNXeβ129Npsin(αXe)sin(αp)γXeγpe−tdisc/T∗2,Xee−tdisc/T∗2,p S

Xe

S

p

=

P

Xe

P

p

N

Xe

β

129

N

p

sin

(

α

Xe

)

sin

(

α

p

)

γ

Xe

γ

p

e

t

d

i

s

c

/

T

2

,

X

e

*

e

t

d

i

s

c

/

T

2

,

p

*

where S is the free induction decay amplitude (or the integral of the fast Fourier transform of the signal), N is the number of nuclei in the measured volume, β 129 is the isotopic abundance of the 129 Xe, α is the excitation flip angle, and γ is the nuclear gyromagnetic ratio. The last correction factor in Eq 2 is to account for T∗2 T

2

* losses during the delay time before the start of acquisition, necessary because of coil coupling. It extrapolates the signal value back to time t aq = 0 and it is significant because of different T∗2 T

2

* values between proton and xenon. For protons, the polarization is given by the Boltzmann equilibrium of the spins inside the low magnetic field needed to observe a 33.7 kHz NMR frequency of 7.92 gauss ( P = 2.6 × 10 –9 at room temperature). To obtain a good SNR for the proton signal, long acquisition times are required (usually 1,024 averages with a 15-second repetition time between the shots). The number of xenon atoms was calculated based on the ideal gas law. Xenon files are acquired using four averages with 90° flip angle. The repetition time is chosen such that the gas inside the down-tube NMR region, depleted of its magnetization by the measurement, is completely replaced by polarized gas mixture (typically 20 seconds).

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Transportation System

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Figure 6, (a) Frozen xenon delivery system: large cryobiologic storage system with a permanent magnet box to assure a 0.1-T holding field during delivery. (b) Gas phase transport system: large case enclosing the solenoid coil with the delivery cell of 1 L in the center. The coil can be powered from the wall, car (cigarette lighter socket), or by an internal rechargeable battery.

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Results

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Figure 7, Largest 129 Xe polarization numbers achieved during preliminary tests at optimum found operating parameters of 160°C; 500 torr total pressure; 1,350 sccm total flow; and ∼125 torr nitrogen partial pressure. Polarization numbers are fitted with an empirical exponential curve.

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Xenon Polarization

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Freeze-Thaw Polarization Recovery

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Gas Phase Relaxation Measurements

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Production Rates

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P=P0(1−e−tacc/T1)T1tacc P

=

P

0

(

1

e

t

a

c

c

/

T

1

)

T

1

t

a

c

c

where T 1 is the longitudinal relaxation time in frozen state and t acc is the accumulation time. If the output gas polarization can be estimated as a function of its flow rate, P 0 = P 0 (Φ), then one can calculate the optimum xenon flow rate and the required time to be produced. We fitted the experimental data shown in Fig 7 with an exponential function resulting in:

P0(Φ)=18.25+52.51×e−Φ(sih)2.32(%) P

0

(

Φ

)

=

18.25

+

52.51

×

e

Φ

(

sih

)

2.32

(

%

)

Figure 8 shows the xenon production map generated by implementing Eq 4 into Eq 3 . The displayed polarization numbers do not include any losses from the freeze-thaw cycle and they are characteristic for 2.5 hours of xenon relaxation time in a frozen state. The map shows that our system can produce a half-liter of xenon with polarization ∼50% or 1 L of xenon with polarization ∼44%.

Figure 8, Polarized xenon production map considering a relaxation time in frozen state of 2.5 hours and no polarization loss during the freeze-thaw cycle. Numbers shown on the contour lines represent final polarization expressed in percent.

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Polarizer Relocation

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Compact Prototype System

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Figure 9, Prototype of compact xenon polarizer: (a) computer-assisted diagnosis drawing of the system components. Labeled components are: 1) coil tower surrounding the oven and the column, 2) laser and optics box, 3) double mirror box for laser beam deviation into the polarizing column, 4) nuclear magnetic resonance permanent magnet setup, 5) gas flow panel, 6) dedicated controlling and monitoring computer, 7) vacuum pump, 8) laser chiller; (b) same as previous, only that column is shown in servicing mode; (c) real system under testing and refinement.

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Discussion

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