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Sodium MRI of a Human Transplanted Kidney

Rationale and Objectives

Sodium magnetic resonance imaging (MRI) of the kidneys has been used to spatially map areas of sodium-concentrating activity and to quantify the corticomedullary sodium gradient in various physiologic and pathophysiologic conditions. In this case study, sodium MRI of a clinically well-functioning transplanted kidney was performed to determine whether its sodium gradient could be detected and quantified using this method.

Materials and Methods

Sodium MRI was performed on a 3T scanner with a commercial rectangular sodium surface coil placed on the lower abdomen over the palpable transplanted kidney. A three-dimensional gradient echo sequence, modified for multinuclear imaging, was applied to acquire 23 Na images.

Results

Five main renal pyramids within the medulla were detected, and the corticomedullary sodium gradient was quantified in each renal pyramid by both region of interest–based and pixel-by-pixel analyses, resulting in a mean medulla/cortex signal-to-noise ratio of 1.8 ± 0.1 ( n = 5) and a mean linear increase slope of 1.1 ± 0.1 relative arbitrary units per mm ( n = 5).

Conclusions

The feasibility and usability of 23 Na MRI of a human renal allograft was demonstrated. Further studies are required to determine the clinical significance of this technique in the follow-up of patients after renal transplantation.

Sodium magnetic resonance imaging (MRI) is a noninvasive tool to map the sodium spatial distribution in the imaged organ and has been described for various targeted applications . In the kidney, sodium MRI may have a unique role since a well functioning kidney will maintain a corticomedullary sodium gradient to keep body fluids and electrolytes in homeostasis .

In recent years, there have been several reports of using 23 Na MRI to noninvasively measure the corticomedullary sodium gradient in animal models . It has been shown that the sodium gradient measured by 23 Na MRI was linear, and sensitive to physiologic and pathophysiologic changes as diuresis , hydronephrosis , and acute tubular necrosis .

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Materials and methods

Subject

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MRI Acquisition

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Data Processing and Image Analysis

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Results

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Figure 1, (a) A sagittal sodium reconstruction of two combined central slices of the three-dimensional 23 Na image acquired at 3T in 25 minutes. The transplanted kidney is clearly observed against the abdominal wall, showing the high-intensity medullary areas in the renal parenchyma. Data were acquired using a sodium surface coil and applying a three-dimensional gradient echo sequence tailored for sodium imaging with repetition time/echo time = 30/1.8 ms (partial Fourier echo), field of view = 38 × 38 × 24 cm, matrix = 128 × 128 × 16. (b) A coronal reconstruction of the cropped three-dimensional renal parenchyma, summed into a single coronal slice (using the inherent image processing tools of the scanner). This view facilitates the three-dimensional spatial localization of the main medullary areas.

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Figure 2, A plot of the sodium signal-to-noise ratio (SNR) along one of the corticomedullary axes, obtained from a sagittal sodium image acquired as described in the Methods section. The observed increase in sodium SNR along the corticomedullary axis was linear (in this example r 2 > 0.99). The error bars are based on the uncertainties present from the SNR measured at each point.

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Discussion

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Table 1

A Comparison between the Transplanted Kidney Sodium Data and Previously Studied Intact Kidneys

Transplanted Kidney ( n = 1) Intact Kidneys ( n = 6) SNR: whole kidney 19.6 18.2 ± 2.5 SNR: cortex 12.9 11.8 ± 1.3 SNR: medulla 28.3 28.3 ± 3.3 Number of renal pyramids 5.0 5.0 ± 0.2 Sodium gradient: Medulla/cortex ROI ratio 1.8 2.4 ± 0.1 Linear slope (Rel.u./mm) 1.1 ( r 2 = 0.98) 1.6 ± 0.2 ( r 2 = 0.96 ± 0.01)

SNR, signal-to-noise ratio; ROI, region of interest.

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Acknowledgments

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References

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